The paper deals with the fabrication of Montmorillonite impregnated cellulose acetate nanofiber membrane by electrospinning method and the application of the membrane for removal of widely used Ciprofloxacin antibiotic from wastewater. The membrane fabrication conditions were optimized keeping the physical characteristics such as fabrication, strength as basic criteria and its removal efficiency for Ciprofloxacin from wastewater. Very fine nanofiber membranes were formed with an approximate size of less than 100 nm. The adsorption experiments were conducted with initial CIP concentration of 10 mg/L, adsorbent dose of 1 g/L, 3 g/L and 5 g/L at a pH of 4. The contact time was 5 h. The adsorptive removal efficiencies were varying from 27% to more than 90% depending upon the combination used.