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Study of antimicrobial activities of green synthesized silver nanoparticles

Authors : R. Khandelwal *a, S. K. Arora b, D. M. Phase c, Arun Pareek b and Ravi Kant d



Green synthesis is the pollution free synthesis process, safe for the environment and cost-effective too. In the present paper we repot our studies on the green synthesis of silver nanoparticles using seed extracts of Phaseolus vulgarisTrachyspermum ammi and Trigonella Foenum-Graecum and their applications for antimicrobial activity. The preliminary confirmation of the nanoparticles formation was done by visual observation then by UV-Visible absorption spectrum, which showed SPR peak in the range of 400–450 nm, which is characteristic peak of silver nanoparticles. To confirm the role of phytochemicals in the stabilization of silver nanoparticles as capping agents, the Fourier Transform Infrared Spectroscopy (FTIR) analysis carried out which show the presence of proteins with the synthesized silver nanoparticles. The plant extract was found to exhibit strong potential for rapid reduction of silver ions. The synthesized nanoparticles are then analyzed for their potency in medical applications for antimicrobial activity. Green synthesis of nanoparticles is proving to be more effective in medicinal applications than the bare metal nanoparticles since the biomass coatings on them act synergistically with the metal nanoparticles.


Green synthesis, AgNPs, XRD, UV-Visible absorption spectroscopy, antimicrobial activity