The effluent discharge from industries such as textile, pharmaceutical, leather dyeing, and petroleum containing different dyes is one of the major sources of groundwater contamination. Dyes are toxic, mutagenic, and carcinogenic in nature, and adversely affect human beings and aquatic life. The remediation of dye is one of the major challenges to the researchers. In this work, an attempt has been made to solve the above concern. The potential bacterial species were isolated from dye contaminated site and used in the removal of Methylene blue (MB) dye. The most affecting process variable including process time, dye concentration, and pH were optimized using a central composite design (CCD) of response surface methodology (RSM) and obtained to be 4.0 days, 50 mg/L, and 7.0, respectively. The dye removal efficiency was found to be 78.2% at optimum conditions.