The aim of the study was to study the metal ion content, anti-diarrheal, anti-ulcer, antioxidants and antibacterial activities of the Thymus linearis Benth crude extract collected from Nepal. The T. linearis crude methanol extract revealed highest amount of total phenolic content (TPC) 211.709±2.51 mg GAE/g DW and total flavonoid content (TFC) 29.12±0.65 mg QUE/g DW, respectively. Furthermore, highest antioxidant activities were also recorded in methanol extract with IC50 value of 80.77±0.99 g/ml. Our result also revealed that the methanol crude extract showed the highest antibacterial and antioxidant activities followed by acetone, water and hexane extract. The crude water extracts revealed highest anti-diarrheal and anti-ulcer properties with 68.75% and 66% of inhibition, respectively in the mouse model. The metal ion analysis of the plant extracts was carried out using AAS. Results revealed that T. linearis is a rich source of iron, sodium, and potassium. In conclusion T. linearis Benth possess potential compounds like polyphenols, flavonoid, tannins, quinone, cardioglycosides as well as the rich source of essential elements which are the reasons behind the anti-ulcer, anti-diarrheal, antioxidant and anti-microbial property of the herbal extract.